The recombinant Vesicular Stomatitis Virus (rVSV-ΔG) reverse genetics pseudotype system is widely used to generate rVSV particles that express the surface glycoprotein protein of a heterologous virus. This is a particularly powerful tool to study viruses that require high level biosafety containment (BSL-3 and BSL-4) such as Coronaviruses and Filoviruses1,2,3, as it generates virus that is restricted to a single round of replication, making them BSL-2 level compatible.

产品类别: Virus

血清型: VSV Indiana / Ebola Makona

Synonyms: EBOV-VSV, Ebola virus pseudotype, rVSV-EBOV, rVSV-Ebola, Ebola virus, VSV-EBOV

Biosafety Level: BSL-2

应用: Screening antibodies or drugs for anti-Ebola neutralization activity, monitoring Ebola virus entry into target cells.

储存条件: -80C. Multiple freeze thaw cycles will reduce titer.

运输条件: Dry Ice

滴度:  N/A

包装尺寸: A single vial can be used for 96 reactions. Please contact services@eliteimmune.com for protocol.

目的用途: This product is for research use only. It is not intended for human, therapeutic, or diagnostic use. Buyer may not modify, sell, or transfer product for commercial use without written permission from Eliteimmune.

参考文献:

1.Howell, K.A., et al., Cooperativity Enables Non-Neutralizing Antibodies to Neutralize Ebolavirus. Cell Reports, 2017.

2.Zhao et al. Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability Cell. 2017.

3.Whitt, M.A., Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.

Alternative Names:

EBOV-VSV, Ebola virus pseudotype, rVSV-EBOV, rVSV-Ebola, Ebola virus, VSV-EBOV