Catalog Number: F300-007

Lot: 86532

Description:

The recombinant Vesicular Stomatitis Virus (rVSV-ΔG) reverse genetics pseudotype system is widely used to generate rVSV particles that express the surface glycoprotein protein of a heterologous virus. This is a particularly powerful tool to study viruses that require high level biosafety containment (BSL-3 and BSL-4) such as Coronaviruses and Filoviruses1,2,3, as it generates virus that is restricted to a single round of replication, making them BSL-2 level compatible.

Product Type: Virus

Serotype: VSV Indiana / Marburg RAVN

Synonyms: RAVN-VSV, Marburg virus pseudotype, rVSV-RAVV, rVSV-RAVN, Maburg virus, VSV-RAVV

Biosafety Level: BSL-2

Applications: Screening antibodies or drugs for anti-Ebola neutralization activity, monitoring Ebola virus entry into target cells.

Titer:  Will be provided with packing list

Size: A single vial can be used for 96 reactions. Please contact services@eliteimmune.com for protocol.

Store: -80°C. Multiple freeze thaw cycles will reduce titer.

Shipped: Dry Ice

Intended Use: This product is for research use only. It is not intended for human, therapeutic, or diagnostic use. Buyer may not modify, sell, or transfer product for commercial use without written permission from Eliteimmune.

References:

1.      Howell, K.A., et al., Cooperativity Enables Non-Neutralizing Antibodies to Neutralize Ebolavirus. Cell Reports, 2017.

2.     Zhao et al. Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability Cell. 2017.

3.     Whitt, M.A., Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. J. Virol. Methods, 2010. 169(2): p. 365-74.

Alternative Names:

RAVN-VSV, Marburg virus pseudotype, rVSV-RAVV, rVSV-RAVN, Maburg virus, VSV-RAVV