Catalog Number: C101-005

Lot: 61718

Description:

MERS-CoV, also known as NCoV (novel coronavirus) is a positive sense single stranded RNA virus that causes Middle East respiratory syndrome (MERS). This respiratory virus was first identified in June 2012 in Jeddah, Saudi Arabia¹, and while most cases have occurred in the Arabian Peninsula, is spread to 27 countries have reported cases since 2012, leading to 858 known deaths due to the infection and related complications^1,2,3. MERS-CoV expresses a trimeric spike glycoprotein on the surface the virus, which is comprised of S1 and S2 domains. Within the S1 domain is the receptor binding domain (RBD) that is responsible for binding to the host receptor DPP4⁴, allowing the virus to enter permissive cells. The predicted molecular weight is 150 kDa. The observed molecular weight may differ from predicted due to post translational modifications.

Product Type: Recombinant Protein

Strain: MERS Jordan

Synonyms: MERS-CoV, MERS Coronavirus, MERS-CoV Spike, Coronavirus Spike Protein, MERS, Middle East respiratory syndrome coronavirus, EMC/2012

Expression Host: Drosophila (S2)

Tag: C- terminal Strep-His-Avi

Purity: >95% by SDS-PAGE

Biosafety Level: BSL-2

Applications: ELISA, Western Blot, SDS-PAGE, immunogen. Specific conditions should be optimized by end user.

Formulation: PBS

Storage: 4°C for immediate use, -80°C for long-term storage. Avoid repeated freeze thaw cycles.

Shipped: Dry Ice

Intended Use: For research use only. Not intended for human, therapeutic, or diagnostic use. Buyer may not modify, sell, or transfer product for commercial use without written permission from Eliteimmune.

References:

1.      Bermingham A, Chand MA, Brown CS, et al. Severe respiratory illness caused by a novel coronavirus, in a patient transferred to the United Kingdom from the Middle East, September 2012. Euro Surveill. 2012

2.     Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012

3.     de Groot RJ, Baker SC, Baric RS, et al. Middle East Respiratory Syndrome Coronavirus (MERS-CoV); Announcement of the Coronavirus Study Group. J Virol. 2013

4.     Raj VS et al. Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC. Nature. 2013.  

Alternative Names:

MERS-CoV, MERS Coronavirus, MERS-CoV Spike, Coronavirus Spike Protein, MERS, Middle East respiratory