Catalog Number: C100-003 

Product Type: Recombinant Protein 

Strain: Wuhan-Hu-1 (GenBank: MN908947) 

Expression Host: CHO cells 

Tag: C- terminal Histidine tag (8x-His)   

Purity: >95% by SDS-PAGE Biosafety 

Level: BSL-1 Applications: ELISA, Western Blot, SDS-PAGE, immunogen. Specific conditions should be optimized by end user.

Formulation: PBS

Storage: 4°C for immediate use, -80°C for long-term storage. Avoid repeated freeze thaw cycles. 

Shipped: Dry Ice

Synonyms: SARS-CoV2 receptor binding domain, 2019-nCoV RBD, 2019-nCoV receptor binding domain, SARS-CoV-2 Spike protein, SARS-CoV-2 S1, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) 

Description: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a positive sense RNA virus responsible for the COVID-19 pandemic. SARS-CoV-2 expresses a trimeric spike glycoprotein on the surface the virus, which is comprised of S1 and S2 domains. Within the S1 domain is the receptor binding domain (RBD) that is responsible for binding to the host receptor angiotensin-converting enzyme 2 (ACE2), and allowing the virus to enter permissive cells^1,2. Mutations within the RBD can also lead to decreased affinity of neutralizing antibodies to binding to the virus³.

Notes: Predicted molecular weight is 33.2 kDa. The observed molecular weight may differ from predicted due to post translational modifications. 

References: 

1. Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. Yan et al. Science 2020.

2. Structure of SARS-CoV-2 Spike receptor-binding domain bound to the ACE2 receptor. Lan J et al. Nature 2020.

3. Greaney et al. Comprehensive mapping of mutations in the SARS-CoV-2 receptor-binding domain that affect recognition by polyclonal human plasma antibodies., Cell Host Microbe. 2021 Intended Use: For research use only. Not intended for human, therapeutic, or diagnostic use. Buyer may not modify, sell, or transfer product for commercial use without written permission from Eliteimmune.

Alternative Names:

SARS-CoV2 receptor binding domain, 2019-nCoV RBD, 2019-nCoV receptor binding domain, SARS-CoV-2 Spike protein, SARS-CoV-2 S1, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)